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Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies

Kumar, Pramod
; 
Satyam, Abhigyan
; 
Fan, Xingliang
; 
Collin, Estelle
; 
Rochev, Yury
; 
Rodriguez, Brian J.
; 
Gorelov, Alexander
; 
Dillon, Simon
; 
Joshi, Lokesh
; 
Raghunath, Michael
... show 2 more
Identifiers
http://hdl.handle.net/10379/12337
 https://doi.org/10.13025/28923
Publication Date
2015-03-04
Keywords
stem-cell culture, adult arterial revascularization, human corneal fibroblasts, growth-factor regulation, fetal bovine serum, keratocyte phenotype, collagen matrices, conditioned media, epithelial-cells, ascorbic-acid
Type
Article
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Citation
Kumar, Pramod; Satyam, Abhigyan; Fan, Xingliang; Collin, Estelle; Rochev, Yury; Rodriguez, Brian J. Gorelov, Alexander; Dillon, Simon; Joshi, Lokesh; Raghunath, Michael; Pandit, Abhay; Zeugolis, Dimitrios I. (2015). Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports 5 ,
Abstract
Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.
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Publisher
Springer Nature
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)