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An essential role for coagulase instaphylococcus aureusbiofilm development reveals new therapeutic possibilities for device-related infections

Zapotoczna, Marta
; 
McCarthy, Hannah
; 
Rudkin, Justine K.
; 
O'Gara, James P.
; 
O'Neill, Eoghan
Identifiers
http://hdl.handle.net/10379/14513
 https://doi.org/10.13025/28927
Publication Date
2015-06-04
Keywords
staphylococcus, biofilm, infection, coagulase, antimicrobial, susceptibility, fibronectin-binding proteins, gene-expression, immune evasion, virulence, mechanisms, fibrinogen, sara, pathogenicity, exoproteins, epidermidis
Type
Article
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Citation
Zapotoczna, Marta; McCarthy, Hannah; Rudkin, Justine K. O'Gara, James P.; O'Neill, Eoghan (2015). An essential role for coagulase instaphylococcus aureusbiofilm development reveals new therapeutic possibilities for device-related infections. Journal of Infectious Diseases 212 (12), 1883-1893
Abstract
High-level resistance to antimicrobial drugs is a major factor in the pathogenesis of chronic Staphylococcus aureus biofilm-associated, medical device-related infections. Antimicrobial susceptibility analysis revealed that biofilms grown for <= 24 hours on biomaterials conditioned with human plasma under venous shear in iron-free cell culture medium were significantly more susceptible to antistaphylococcal antibiotics. Biofilms formed under these physiologically relevant conditions were regulated by SaeRS and dependent on coagulase-catalyzed conversion of fibrinogen into fibrin. In contrast, SarA-regulated biofilms formed on uncoated polystyrene in nutrient-rich bacteriological medium were mediated by the previously characterized biofilm factors poly-N-acetyl glucosamine, fibronectin-binding proteins, or autolytic activity and were antibiotic resistant. Coagulase-mediated biofilms exhibited increased antimicrobial resistance over time (>48 hours) but were always susceptible to dispersal by the fibrinolytic enzymes plasmin or nattokinase. Biofilms recovered from infected central venous catheters in a rat model of device-related infection were dispersed by nattokinase, supporting the important role of the biofilm phenotype and identifying a potentially new therapeutic approach with anti-microbials and fibrinolytic drugs, particularly during the early stages of device-related infection.
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Publisher
Oxford University Press (OUP)
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Rights
Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (2)