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Pediocin a, a bacteriocin produced by pediococcus pentosaceus fbb61

Piva, A.
; 
Headon, D. R.
Identifiers
http://hdl.handle.net/10379/9782
https://doi.org/10.13025/24614
Repository DOI
10.1099/00221287-140-4-697
Publication Date
1994-04-01
Keywords
pediocin a, bacteriocin, pediococcus pentosaceus, food microbiology, electroendosmotic preparative electrophoresis, amino-acid-sequence, listeria-monocytogenes, lactococcus-lactis, acidilactici-h, purification, inhibition, ach
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Article
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Citation
Piva, A. Headon, D. R. (1994). Pediocin a, a bacteriocin produced by pediococcus pentosaceus fbb61. Microbiology 140 , 697-702
Abstract
Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61, was purified and partially characterized. The purification was achieved by dialysis against BEG 20000, butanol extraction and electroendosmotic preparative electrophoresis. The protocol led to a 7843-fold increase in the specific activity, with 3.9% activity recovered. SDS-PAGE of pediocin A resulted in a single 80 kDa protein band. The antimicrobial compound was sensitive to proteolytic enzymes and heat (10 min at 100 degrees C). It exhibited inhibition against species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Listeria and Clostridium.
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Publisher
Microbiology Society
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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Externally hosted open access publications with University of Galway authors (1)