Negative energy balance in dairy cows is associated with specific changes in igf-binding protein expression in the oviduct
Fenwick, M A Llewellyn, S Fitzpatrick, R Kenny, D A Murphy, J J Patton, J Wathes, D C
Fenwick, M A
Llewellyn, S
Fitzpatrick, R
Kenny, D A
Murphy, J J
Patton, J
Wathes, D C
Identifiers
http://hdl.handle.net/10379/11450
https://doi.org/10.13025/29040
https://doi.org/10.13025/29040
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Publication Date
2007-12-25
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Article
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Fenwick, M A; Llewellyn, S; Fitzpatrick, R; Kenny, D A; Murphy, J J; Patton, J; Wathes, D C (2007). Negative energy balance in dairy cows is associated with specific changes in igf-binding protein expression in the oviduct. Reproduction 135 (1), 63-75
Abstract
Negative energy balance (NEB) during early lactation in dairy cows leads to an altered metabolic state that has major effects on the production of IGF family members. Low IGF-I concentrations are associated with poor fertility and therefore we aimed to determine whether NEB exerts a direct effect on IGF expression in the postpartum oviduct. Multiparous Holstein cows were allocated to two treatments (each n=6) designed using differential feeding and milking regimes to produce either mild NEB (MNEB) or severe NEB (SNEB). Animals were slaughtered in week 2 of lactation when divergent metabolic profiles were evident. Oviducts were collected for RNA analysis by real-time RT-PCR and in situ hybridisation. Quantitative measures in oviduct gene expression were obtained for all members of the IGF family (IGF-I/II, IGF-binding proteins (IGFBP) 1-6 and receptors for IGF types 1 and 2), insulin A/B, GH, glucocorticoid and oestrogen alpha/beta. Expression of IGFBP-2 and IGFBP-6 (both of which have a high affinity for IGF-II) was decreased in SNEB relative to MNEB (P<0.05). No other gene was altered by NEB, but IGF-II, IGFBP-3, IGFBP-5 and IGFBP-6 all showed differential expression in different regions of the oviduct. These results indicate that, in addition to low circulating IGF-I after calving, NEB may also influence IGF availability in the oviduct indirectly through changes in specific IGFBP expression. It is possible that the predicted increased signalling by IGF-II may perturb embryo development, contributing to the high rates of embryonic mortality in dairy cows.
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BioScientifica
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Attribution-NonCommercial-NoDerivs 3.0 Ireland