Developing a three dimensional (3D) in vitro model of adrenocortical carcinoma (ACC)
Ribes Martinez, Eduardo
Ribes Martinez, Eduardo
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Identifiers
http://hdl.handle.net/10379/18133
https://doi.org/10.13025/18054
https://doi.org/10.13025/18054
Repository DOI
Publication Date
2024-04-05
Type
Thesis
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Abstract
Adrenocortical carcinoma (ACC) is a rare orphan disease and a rare cancer which carries a poor prognosis. The only licensed pharmacological treatment is mitotane, an insecticide. Typically, 2D cell culture models have been used to study ACC and represent the best available disease models for mechanistic insight of disease and therapeutics. However, these models have limited representation of disease in humans. Moreover, the rare nature of disease translates to a lack of human tissue specimens for research and culture, again limiting the investigation of disease mechanism in the context of ACC. As such all-cell lines and animal models are limited in their representation of human disease and hence multiple models are usually necessary to design investigative approaches to disease which help thoroughly understand and study the biology and propagation of this cancer. In the current thesis of work, we developed and characterize a spheroid model for primary tumour cell line, H295R and a metastatic cell line, MUC-1, showing a steroidogenic enzymatic cell profile. Additionally, we develop a collagen scaffold 3D model of H295R and MUC-1 that attempts to mimic the complexity of the extracellular matrix (ECM) supporting cell viability, metabolic activity, steroids production. Despite the lack of experimental replicates, altogether represents a preliminary data for a model to study ACC which retains its phenotype and steroidogenic properties.
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Publisher
NUI Galway